• Image
  • Image
  • Image
  • Image

Vasculata 2018 Workshops

Vasculata 2018

July 23-26, 2018
St. Louis, MO


Workshops will be held on Wednesday, July 25 (most workshops will begin at 10:00am or 1:00pm)

Workshops are limited to six attendees unless otherwise noted, please check each workshop (some are less than six).  There is an additional fee of $25 for each workshop. The Writing Workshop is limited to 50 people and is free.

Transportation will be provided for those attending a workshop at the University of Missouri-Columbia

Workshops will fill up fast - so, register early in order to secure your place.

University of Missouri Workshops

Skeletal Muscle Microcirculation

Instructor: Dr. Steven Segal

Description: This workshop will demonstrate the gluteus maximus muscle preparation for imaging and evaluating the microcirculation of skeletal muscle. The workshop will include an overview of microvascular architecture in skeletal muscle, evaluation of endothelium-dependent vasodilation, adrenergic vasoconstriction, vasomotor tone and microvascular perfusion.

Schedule: 1:00-5:00pm

*This workshop can accommodate only 3 attendees

Isolated Vessels/Pressure Myography

Instructors: Drs. Michael Davis and Erika Boerman

Description: This workshop will demonstrate the techniques used to microdissect, cannulate, clean and pressurize small arterioles and lymphatic vessels for ex vivo studies. The workshop will include a discussion of methods for manufacturing glass micropipettes and sharpening microsurgical instruments, as well as a demonstration of different systems that can be used to control pressure during an experiment. Opportunities will be available for hands-on experience in dissection and cannulation for each student.

Schedule: 1:00-5:00pm

*This session is limited to only 3 attendees

Permeability Assessments

Instructors: Dr. Virginia H. Huxley and JianJie Wang

Description: The techniques for making quantitative measures of in vivo microvessel solute permeability and/or hydraulic conductivity will be discussed, demonstrated and opportunities given for hands-on experience by the participants. The workshop will also include a discussion of methods for manufacturing glass microperfusion pipettes and preparation of the solutions of solutes, as well as a demonstration of different systems that can be used to control perfusion during an experiment.

Schedule: 1:00-5:00pm

*This session is limited to only 3 attendees


Washington University Workshops

2-Photon Imaging of the Blood and Lymphatic Vasculature

Instructor: Dr. Bernd Zinselmeyer

Description: This workshop will demonstrate the preparation of popliteal vessels in mice for lymphatic and blood vascular imaging.

10:00-11:20 Demonstration of intravital preparation set up
11:20 – 12:30 lunch break
12:30 – 2:00 Fluorescence imaging lecture including concepts underlying 2-photon microscopy
2:00-2:30 coffee break
2:30 – 5 p.m. live imaging and analysis at the microscope and analysis station

Location: BJCIH 8th floor, Washington University School of Medicine campus

Embryoid body differentiation and vascular sprouting

Instructor: Dr. Kyunghee Choi

Description: This workshop will introduce the study of embryonic stem cell differentiation into embryoid bodies. Demonstrations will be established to illustrate how the embryoid body system can be used to study vascular sprouting and screen for mediators of this process. It will also demonstrate the use of embryoid bodies for cardiac myocyte differentiation and function. Hands-on experience in seeding embryoid bodies in matrigel and initiating differentiation will be included in the workshop.

1 – 3 p.m. Group 1, EB seeding and differentiation; EB sprouting demo; Cardiac myocyte demo.
3:00 – 3:30 Lecture on ES cells and embryoid bodies.
3:30 – 5:30 Group 2, Group 1, EB seeding and differentiation; EB sprouting demo; Cardiac myocyte demo.

Location: 8th floor, BJCIH; Washington University School of Medicine

*This workshop can accommodate 10 attendees

Mechanical Testing in the Vasculature

Instructor: Dr. Jessica Wagenseil

Description: This workshop will provide hands on training in biaxial mechanical testing of mouse carotid arteries. The workshop can accommodate 6 students (3 each on two testing machines).

Mouse carotid arteries will be dissected ahead of time and stored in refrigerated saline before use. Participants will mount an artery on a commercial pressure myograph machine (DMT). Participants will inflate the artery to supra-physiologic pressures at different axial stretches and stretch the artery to supra-physiologic axial stretches at different pressures while recording pressure, outer diameter, axial force, and axial length. After testing, participants will cut cross-sections of the artery for measuring the unloaded dimensions and calculating residual stresses. Considerations for determining stress, strain, stiffness, modulus, and energy storage capacity from the testing data will be discussed.

Schedule: 2:00-5:00pm

Location: Urbauer 16 - Danforth campus

Cerebral Vasculature and Vascular Casting

Instructor: Dr. Andrew Elvington, Missouri Baptist University

Description: More information is coming soon


DropSeq and InDrop Workshop

Instructor: Dr. Benjamin Humphreys

Description: More information is coming soon


Metabolic Techniques in the Cardiovascular and Vascular Systems

Instructor: Dr. Joel Schilling

Description: More information is coming soon


Noninvasive Imaging of the Vasculature using Quantitative Imaging Modalities

Leaders: Monica Shokeen, Ph.D., Optical Imaging Lab, Radiology; and Dana R. Abendschein, Ph.D., Internal Medicine/Cardiology, Washington University School of Medicine in St. Louis

10-11: Measuring Angiogenesis with PET/CT and Complex Atherosclerosis/Vascular Injury with PET/MRI
and Optical Imaging – Dana
Animal models of occlusion and hind limb ischemia will be discussed/demonstrated
11-12: Assessing Vascularity and Bone Marrow Composition in Multiple Myeloma with Optical, Nuclear and Magnetic Resonance Imaging – Monica
Pros and cons of various animal models for studying hematological malignancies will be discussed
12-1: Lunch Break
1-2:30: Field Trip to MicroPET/MR and Optical facilities in MIR and Biomedical Couch Building (Optical Radiology Laboratory)
2:45-5:00: We will include hands on exercises in image analysis. View microPET image analysis in Optical Imaging Lab, Couch Building and/or have additional topical discussions with WU investigators. Possibilities include alpha v beta 3 targeting for imaging (Dr. Weilbaecher), brain imaging (Dr. Corbo), Culver group for brain vasculature imaging (oxy/deoxy) and/or other applications as participants have interest (Dana or Monica to facilitate connections).

*This workshop can accommodate 15 attendees

Zebrafish Workshop

Instructors: Drs. Saulius Sumanas and Lila Solnica-Krezel

Description: More information is coming soon


Flow cytometric analysis of leukocyte populations isolated from atherosclerotic aorta

Instructor: Jesse Williams, PhD, Dept. of Pathology and Immunology, Washington University School of Medicine

10-11:30 am: Demonstration and hands-on experience in isolation of aorta; including ascending, descending, abdominal, and carotid branches from atherosclerotic mice.
11:30-1:00 pm: Digestion protocol to separate aortic adventitia from the intima/media, followed by single cell suspension preparation
1:00-2:30 pm: Lunch Break
2:30-3:30 pm: Flow cytometric leukocyte staining and introduction to flow cytometry
3:30-5:30 pm: Field trip: Washington University Flow Cytometry Core, group training on flow cytometer (BD LSRFORTESSA X-20) setup, compensation (FACS DIVA), and gating strategies (FLOWJO).

*This workshop can accommodate 10 attendees

Writing Workshop

Instructor: Dr. Gwen Randolph

Description: Participants will be able to come and go as they please. If you are in another workshop that is perhaps just a half day, you can participate in this workshop the other half (except for those going to the University of Missouri).

Schedule: 10:00am - 5:00pm